Enzymic hydrolysis of adenosine phosphates by cobra venom.
نویسندگان
چکیده
We wish to thank Mr G. T. Iles of Belle Vue, Manchester, for supplying us with the sample of python fat used in this investigation, and for the information concerning the animal; also Prof. J. W. Cook, F.R.S., for drawing our attention to the work on bile salts; the University of Glasgow for a scholarship held by one of us (R.P.P.) and Imperial Chemical Industries Ltd., for a grant towards the purchase of apparatus.
منابع مشابه
The stereochemical course of hydrolysis catalysed by snake venom 5'-nucleotide phosphodiesterase.
Adenosine 5'-(S)-[16O,17O,18O]phosphate was pyrophosphorylated by the combined action of adenylate kinase and pyruvate kinase. The isotopomers of adenosine 5'-[alpha-16O,17O,18O]triphosphate were hydrolysed by venom 5'-nucleotide phosphodiesterase (Crotalus adamanteus) in H2(17)O. Analysis by 31P nuclear magnetic resonance spectroscopy of the resulting adenosine 5'-[16O,17O,18O]phosphate, after...
متن کاملIdentification of enzymes and toxins in venoms of Indian cobra and Russell's viper after starch gel electrophoresis.
Snake venoms are known to contain a number of enzymes (l-3). Attempts have been made to correlate the toxicity of snake venoms with their enzymic activity, e.g. between nucleases and toxicity (4), phospholipase A and neurotoxic action of cobra venom (5), and proteolytic and coagulant activity and toxicity (6-8). Most of these studies have been carried out on whole venoms or after elimination of...
متن کاملCHARACTERIZATION OF AN ANTICOAGULANT AGENT IN THE VENOM OF IRANIAN COBRA (NAJA NAJA OXIANA).
Snake venoms contain a rich variety of factors which affect blood coagulation. There have been few reports on the anticoagulant activity of the venom of different cobras, but no such observations have been made on the Iranian cobra, although a procoagulant factor has been described. In this study an anticoagulant factor has been purified from the venom of Naja naja oxiana using gel filtrat...
متن کاملProduction of effective antivenin to treat cobra snake
Conventional treatment of Naja naja oxiana (NNO) envenoming requires large volumes of equine antivenin raised against NNO crude venom. The poor efficiency of this antivenin is assumed to be due to the high molecular weight non-toxic proteins, a strong immunogen, present in the crude venom. These proteins cause depression of antibody formation against the low molecular weight toxic components of...
متن کاملSnake Venom Cytotoxins, Phospholipase A2s, and Zn2+-dependent Metalloproteinases: Mechanisms of Action and Pharmacological Relevance
Snake venom toxins are responsible for causing severe pathology and toxicity following envenomation including necrosis, apoptosis, neurotoxicity, myotoxicity, cardiotoxicity, profuse hemorrhage, and disruption of blood homeostasis. Clinically, snake venom toxins therefore represent a significant hazard to snakebite victims which underscores the need to produce more efficient anti-venom. Some sn...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 54 4 شماره
صفحات -
تاریخ انتشار 1953